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兔子纤溶酶原激活物抑制因子ELISA试剂盒

简要描述:

TUZIXIANRONGMEIYUANJIHUOWUYIZHIYINZI PAI ELISASHIJIHErabbit plasminogen activator inhibitor,PAI ELISASHIJIHE

兔子纤溶酶原激活物抑制因子ELISA试剂盒

网上彩票FANGFAER YONGYUJIANCEWEIZHIKANGTIDEJIANJIEFA:YONGBAOBEIHUANCHONGYEJIANGYIZHIKANGYUANXISHIZHI1~10μg/ml,MEIKONGJIA0.1ml,4℃GUOYE。CIRIXIDI3CI。↓ JIAYIDINGXISHIDEDAIJIANYANGPIN(WEIZHIKANGTI)0.1mlYUSHANGSHUYIBAOBEIZHIFANYINGKONGZHONG,ZHI37℃FUYU1XIAOSHI,XIDI。(TONGSHIZUOKONGBAI、YINXINGJIYANGXINGKONGDUIZHAO) ↓ YUFANYINGKONGZHONG,JIARUXINXIANXISHIDEMEIBIAODIERKANGTI(KANGKANGTI)0.1ml,37℃FUYU30-60FENZHONG,XIDI,zuiHOUYIBIANYONGDDWXIDI。↓ QIYUBUZHOUTONG“SHUANGKANGTIJIAXINFA”DE4、5、6。BIANJIBENDUAN(SAN)SHIJIQICAI1. SHIJI (1)BAOBEIHUANCHONGYE(PH9.60.05MTANSUANYANHUANCHONGYE): NaHCO3 1.59KE NaHCO3 2.93KE JIAZHENGLIUSHUIZHI1000ml(2)XIDIHUANCHONGYE(PH7.4PBS):0.15M KH2PO4 0.2KE Na2HPO4·12H2O 2.9KE NaCl 8.0KE KCl 0.2KE Tween-20 0.05% 0.5ml JIAZHENGLIUSHUIZHI1000ml (3)XISHIYE:NIUXUEQINGBAIDANBAI(BSA)0.1KE JIAXIDIHUANCHONGYEZHI100ml HUOYIYANGXUEQING、TUXUEQINGDENGXUEQINGYUXIDIYEPEICHENG5~10%SHIYONG。(4)ZHONGZHIYE(2M H2SO4):ZHENGLIUSHUI178.3ml,ZHUDIJIARUNONGLIUSUAN(98%)21.7ml。(5)DIWUHUANCHONGYE(PH5.0LINSUANZAONINGMENGSUAN): 0.2MNa2HPO4(28.4KE/L)25.7ml 0.1MNINGMENGSUAN(19.2KE/L)24.3ml JIAZHENGLIUSHUI50ml。(6)TMB(SIJIAJILIANBENAN)SHIYONGYE: TMB(10mg/5mlWUSHUIYICHUN)0.5ml DIWUHUANCHONGYE(PH5.5)10ml 0.75%H2O2 32μl(7)ABTSSHIYONGYE: ABTS 0.5mg DIWUHUANCHONGYE(PH5.5)1ml 3%H2O2 2μl (8)KANGYUAN、KANGTIHEMEIBIAOJIKANGTI。(9)ZHENGCHANGRENXUEQINGHEYANGXINGDUIZHAOXUEQING。2.QICAI:(1)JUBENYIXISULIAOBAN(JIANCHENGMEIBIAOBAN)40KONGHUO96KONG,ELISAJIANCEYI,50μlJI100μlJIAYANGQI,SULIAODITOU,XIAOMAOJIN,XIDIPING。(2)XIAOSHAOBEI、BOLIBANG、SHIGUAN、XIGUANHELIANGTONGDENG。(3)4℃BINGXIANG,37℃FUYUXIANG。

兔子纤溶酶原激活物抑制因子ELISA试剂盒 rabbit plasminogen activator inhibitor,PAI ELISA试剂盒 
 
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